Analysis Note

ControlPositive control: brain.

Negative control: kidney, liver.

Application

Western blot: 1:1,000-1:10,000 using ECL. Recognizes a band of 200-220 kDa.

Immunohistochemistry: 1:1,000-1:10,000 on frozen or paraffin embedded tissue sections. Suggested fixative is 4% paraformaldehyde.

Immunocytochemistry: 1:1,000-1:10,000 on cells in tissue culture fixed for one minute in 3.7% formalin and one minute in -20°C methanol.

Optimal working dilutions must be determined by the end user.

Anti-Neurofilament H Antibody is an antibody against Neurofilament for use in IC, IH, IH(P) & WB.

Immunogen

Purified bovine NF-H.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified chicken IgY in PBS containing 5mM sodium azide.

Format: Purified

Specificity

Recognizes Neurofilament-Heavy (NF-H). The antibody stains sharply defined cytoplasmic filaments in neuronal cell bodies and especially axons.

Target description

200 kDa